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control group were given an equal volume of saline for 28 days.After the last administration all mice were fed with water only, and 24 h s later, mice were anesthetized by intraperitoneal injection of 1% sodium pentobarbital (50 mg/kg)

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Like other NADs, researchers have focused on nanocarriers based on liposomes, chitosan, and other materials to achieve efficient delivery, laying a solid foundation for the clinical application of the CRISPR/Cas9 system